Molecular evolution of the leptin exon 3 in some species of the family CanidaeAgata Chmurzynskaa, Magdalena Zajaca, b and Marek Switonskia
a Department of Genetics and Animal Breeding, August Cieszkowski Agricultural University of Poznan, Poland
b Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
(Received 9 September 2002; accepted 5 February 2003)
The structure of the leptin gene seems to be well conserved. The polymorphism of this gene in four species belonging to the Canidae family (the dog (Canis familiaris) - 16 different breeds, the Chinese racoon dog (Nyctereutes procyonoides procyonoides ), the red fox (Vulpes vulpes) and the arctic fox (Alopex lagopus)) were studied with the use of single strand conformation polymorphism (SSCP), restriction fragment length polymorphism (RFLP) and DNA sequencing techniques. For exon 2, all species presented the same SSCP pattern, while in exon 3 some differences were found. DNA sequencing of exon 3 revealed the presence of six nucleotide substitutions, differentiating the studied species. Three of them cause amino acid substitutions as well. For all dog breeds studied, SSCP patterns were identical.
Key words: leptin / Canidae / polymorphism
Correspondence and reprints: Marek Switonski
© INRA, EDP Sciences 2003