A gene-based radiation hybrid map of chicken microchromosome 14: Comparison to human and alignment to the assembled chicken sequenceMireille Morissona, Sophie Lerouxa, Carine Jiguet-Jiglairea, Sirine Assafb, Frédérique Pitela, Sandrine Lagarrigueb, Suzanne Bardesa, Katia Fevea, Thomas Farauta, Denis Milana and Alain Vignala
a Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, 31326 Castanet-Tolosan, France
b Laboratoire de génétique animale, Institut national de la recherche agronomique, École nationale supérieure d'agronomie de Rennes, 35042 Rennes Cedex, France
(Received 3 May 2004; accepted 11 October 2004)
Abstract - We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. A high rate of intra-chromosomal rearrangements was detected by comparison to the assembled human sequence. Finally, the alignment of the RH map to the assembled chicken sequence showed a small number of discordances, most of which involved the same region of the chromosome spanning between 40.5 and 75.9 cR 6000 on the RH map.
Key words: chicken / comparative mapping / radiation hybrids / microchromosome 14 / intrachromosomal rearrangement
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