| Issue |
Genet. Sel. Evol.
Volume 34, Number 4, July-August 2002
|
|
|---|---|---|
| Page(s) | 521 - 533 | |
| DOI | https://doi.org/10.1051/gse:2002021 | |
Genet. Sel. Evol. 34 (2002) 521-533
DOI: 10.1051/gse:2002021
ChickRH6: a chicken whole-genome radiation hybrid panel
Mireille Morisson, Alexandre Lemière, Sarah Bosc, Maxime Galan, Florence Plisson-Petit, Philippe Pinton, Chantal Delcros, Katia Fève, Frédérique Pitel, Valérie Fillon, Martine Yerle and Alain VignalLaboratoire de génétique cellulaire, Institut national de la recherche agronomique, 31326 Castanet-Tolosan, France
(Received 4 February 2002; accepted 13 May 2002)
Abstract
As a first step towards the development of radiation hybrid maps, we
have produced a radiation hybrid panel in the chicken by fusing female
embryonic diploid fibroblasts irradiated at 6 000 rads with
HPRT-deficient hamster Wg3hCl
2 cells. Due to the low
retention frequency of the chicken fragments, a high number of clones
was produced from which the best ones were selected. Thus, 452 fusion
clones were tested for retention frequencies with a panel of
46 markers. Based on these results, 103 clones with a mean marker
retention of 23.8% were selected for large scale culture to produce
DNA in sufficient quantities for the genotyping of numerous markers.
Retention frequency was tested again with the same 46 markers and the
90 best clones, with a final mean retention frequency of 21.9%, were
selected for the final panel. This panel will be a valuable resource
for fine mapping of markers and genes in the chicken, and will also
help in building BAC contigs.
Key words: chicken / radiation hybrid / mapping
Correspondence and reprints: Mireille Morisson
e-mail: mmorisso@toulouse.inra.fr
© INRA, EDP Sciences 2002