Issue |
Genet. Sel. Evol.
Volume 35, Number 5, September-October 2003
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Page(s) | 559 - 572 | |
DOI | https://doi.org/10.1051/gse:2003039 |
DOI: 10.1051/gse:2003039
AFLP linkage map of the Japanese quail Coturnix japonica
Odile Roussota, Katia Fevea, Florence Plisson-Petita, Frédérique Pitela, Jean-Michel Faureb, Catherine Beaumontb and Alain Vignalaa Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, 31326 Castanet-Tolosan, France
b Station de recherches avicoles, Institut national de la recherche agronomique, 37380 Nouzilly, France
(Received 26 December 2002; accepted 7 April 2003)
Abstract
The quail is a valuable farm and laboratory animal. Yet molecular
information about this species remains scarce. We present here the
first genetic linkage map of the Japanese quail. This comprehensive
map is based solely on amplified fragment length polymorphism (AFLP)
markers. These markers were developed and genotyped in an F2 progeny from a
cross between two lines of quail differing in stress reactivity. A
total of 432 polymorphic AFLP markers were detected with 24
TaqI/EcoRI primer combinations. On average, 18 markers
were produced per primer combination. Two hundred and fifty eight of
the polymorphic markers were assigned to 39 autosomal linkage groups
plus the ZW sex chromosome linkage groups. The linkage groups range
from 2 to 28 markers and from 0.0 to 195.5 cM. The AFLP map covers a
total length of 1516 cM, with an average genetic distance between two
consecutive markers of 7.6 cM. This AFLP map can be enriched with
other marker types, especially mapped chicken genes that will enable
to link the maps of both species and make use of the powerful
comparative mapping approach. This AFLP map of the Japanese quail
already provides an efficient tool for quantitative trait loci (QTL)
mapping.
Key words: Japanese quail / AFLP / genetic map / linkage groups / chromosomes
Correspondence and reprints: Odile Roussot
e-mail: roussot@toulouse.inra.fr
© INRA, EDP Sciences 2003