Genet. Sel. Evol.
Volume 33, Number 4, July-August 2001
|Page(s)||433 - 442|
Genet. Sel. Evol. 33 (2001) 433-442
Genetic diversity in Spanish donkey breeds using microsatellite DNA markersJosé Aranguren-Méndeza, b, Jordi Jordanaa and Mariano Gomezc
a Unitat de Genètica i Millora Animal, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain
b Universidad del Zulia, Facultad de Ciencias Veterinarias, Departamento de Producción Animal, Maracaibo 4001-A, Venezuela
c Servicio de Ganadería, Diputación Foral de Bizkaia, Avda. Lehendakari Aguirre, 9, 2°, 48014 Bilbao, Spain
(Received 27 November 2000; accepted 23 April 2001)
Genetic diversity at 13 equine microsatellite loci was compared in five endangered Spanish donkey breeds: Andaluza, Catalana, Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine microsatellites used in this study were amplified and were polymorphic in the domestic donkey breeds with the exception of HMS1, which was monomorphic, and ASB2, which failed to amplify. Allele number, frequency distributions and mean heterozygosities were very similar among the Spanish donkey breeds. The unbiased expected heterozygosity ( HE) over all the populations varied between 0.637 and 0.684 in this study. The low GST value showed that only 3.6% of the diversity was between breeds (P< 0.01). Significant deviations from Hardy-Weinberg equilibrium were shown for a number of locus-population combinations, except HMS5 that showed agreement in all analysed populations. The cumulative exclusion probability (PE) was 0.999 in each breed, suggesting that the loci would be suitable for donkey parentage testing. The constructed dendrogram from the DA distance matrix showed little differentiation between Spanish breeds, but great differentiation between them and the Moroccan ass and also with the horse, used as an outgroup. These results confirm the potential use of equine microsatellite loci as a tool for genetic studies in domestic donkey populations, which could also be useful for conservation plans.
Key words: donkey / endangered breed / microsatellite / diversity / genetic variability
Correspondence and reprints: Jordi Jordana
© INRA, EDP Sciences 2001