Issue |
Genet. Sel. Evol.
Volume 33, Number 4, July-August 2001
|
|
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Page(s) | 433 - 442 | |
DOI | https://doi.org/10.1051/gse:2001126 |
Genet. Sel. Evol. 33 (2001) 433-442
Genetic diversity in Spanish donkey breeds using microsatellite DNA markers
José Aranguren-Méndeza, b, Jordi Jordanaa and Mariano Gomezca Unitat de Genètica i Millora Animal, Departament de Ciència Animal i dels Aliments, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193-Bellaterra, Barcelona, Spain
b Universidad del Zulia, Facultad de Ciencias Veterinarias, Departamento de Producción Animal, Maracaibo 4001-A, Venezuela
c Servicio de Ganadería, Diputación Foral de Bizkaia, Avda. Lehendakari Aguirre, 9, 2°, 48014 Bilbao, Spain
(Received 27 November 2000; accepted 23 April 2001)
Abstract
Genetic diversity at 13 equine microsatellite loci was compared in
five endangered Spanish donkey breeds: Andaluza, Catalana,
Mallorquina, Encartaciones and Zamorano-Leonesa. All of the equine
microsatellites used in this study were amplified and were polymorphic
in the domestic donkey breeds with the exception of HMS1, which was
monomorphic, and ASB2, which failed to amplify. Allele number,
frequency distributions and mean heterozygosities were very similar
among the Spanish donkey breeds. The unbiased expected heterozygosity
(
HE) over all the populations varied between 0.637
and 0.684 in this study. The low
GST value showed that
only 3.6% of the diversity was between breeds (P< 0.01). Significant
deviations from Hardy-Weinberg equilibrium were shown for a number of
locus-population combinations, except HMS5 that showed agreement in
all analysed populations. The cumulative exclusion probability (PE)
was 0.999 in each breed, suggesting that the loci would be suitable
for donkey parentage testing. The constructed dendrogram from the
DA distance matrix showed little differentiation
between Spanish breeds, but great differentiation between them and the
Moroccan ass and also with the horse, used as an outgroup. These
results confirm the potential use of equine microsatellite loci as a
tool for genetic studies in domestic donkey populations, which could
also be useful for conservation plans.
Key words: donkey / endangered breed / microsatellite / diversity / genetic variability
Correspondence and reprints: Jordi Jordana
e-mail: Jordi.Jordana@uab.es
© INRA, EDP Sciences 2001