Free Access
Genet. Sel. Evol.
Volume 34, Number 2, March-April 2002
Page(s) 255 - 267

Genet. Sel. Evol. 34 (2002) 255-267
DOI: 10.1051/gse:2002007

A higher resolution radiation hybrid map of bovine chromosome 13

Jörg Schläpfera, Nasikhat Stahlberger-Saitbekovaa, Sergio Comincinib, Claude Gaillarda, David Hillsc, Rudolf K. Meyerd, John L. Williamsc, Jim E. Womacke, Andreas Zurbriggend and Gaudenz Dolfa

a  Institute of Animal Genetics, Nutrition and Housing, Faculty of Veterinary Medicine, University of Berne, Bremgartenstrasse 109 a, 3012 Berne, Switzerland
b  Department of Genetics and Microbiology, University of Pavia, Via Ferrata 1, 271000 Pavia, Italy
c  Division of Genomics and Bioinformatics, Roslin Institute (Edinburgh), Roslin, Midlothian, EH25 9PS, UK
d  Institute of Animal Neurology, Faculty of Veterinary Medicine, University of Berne, Bremgartenstrasse 109 a, 3012 Berne, Switzerland
e  Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843, USA

(Received 25 June 2001; accepted 7 December 2001)

In this paper, we present a radiation hybrid framework map of BTA13 composed of nine microsatellite loci, six genes and one EST. The map has been developed using a recently constructed 12'000 rad bovine-hamster whole-genome radiation hybrid panel. Moreover, we present a comprehensive map of BTA13 comprising 72 loci, of which 45 are microsatellites, 20 are genes and seven are ESTs. The map has an estimated length of 2694.7 cR 12'000. The proposed order is in general agreement with published maps of BTA13. Our results only partially support previously published information of five blocks of conserved gene order between cattle and man. We found no evidence for the existence of an HSA20 homologous segment of coding DNA on BTA13 located centromeric of a confirmed HSA10 homologous region. The present map increases the marker density and the marker resolution on BTA13 and enables further insight into the evolutionary development of the chromosome as compared to man.

Key words: bovine chromosome 13 / radiation hybrid / gene mapping / 12'000 rad / comparative mapping

Correspondence and reprints: Jörg Schläpfer

© INRA, EDP Sciences 2002