Issue |
Genet. Sel. Evol.
Volume 34, Number 6, November-December 2002
|
|
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Page(s) | 649 - 656 | |
DOI | https://doi.org/10.1051/gse:2002029 |
DOI: 10.1051/gse:2002029
Localisation of aphidicolin-induced break points in Holstein-Friesian cattle (Bos taurus) using RBG-banding
Viviana Rodrigueza, Silvia Llambía, Alicia Postiglionia, Karina Guevaraa, Gonzalo Rincóna, Gabriel Fernándezb, Beatriz Merniesb and María Victoria Arrugaca Department of Cell and Molecular Biology, Genetic Section, Laboratory of Genetics Analysis in Domestic Animals, Faculty of Veterinary, UDELAR, A. Lasplaces 1550 CP 11600, Montevideo, Uruguay
b Department of Animal Production, Animal breeding Section, Faculty of Veterinary, UDELAR, Uruguay
c Laboratory of Cytogenetics and Molecular Genetics, Faculty of Veterinary, Zaragoza University, Spain
(Received 21 November 2001; accepted 1st July 2002)
Abstract
Fragile sites (FS) seem to play a role in genome instability and may
be involved in karyotype evolution and chromosome aberrations. The
majority of common fragile sites are induced by
aphidicolin. Aphidicolin was used at two different concentrations
(0.15 and 0.30
M) to study the occurrence of FS in the
cattle karyotype. In this paper, a map of aphidicolin induced break
points and fragile sites in cattle chromosomes was constructed. The
statistical analysis indicated that any band with three or more breaks
was significantly damaged (
P < 0.05). According to this result, 30
of the 72 different break points observed were scored as fragile
sites. The Pearson correlation test showed a positive association
between chromosome length and the number of fragile sites
(
r=0.54). On the contrary, 21 FS were identified on negative R bands
while 9 FS were located on positive R bands.
Key words: cattle / chromosome / fragile sites / aphidicolin
Correspondence and reprints: Silvia Llambí
e-mail: sllambi@adinet.com.uy; alipos@adinet.com.uy
© INRA, EDP Sciences 2002