Issue |
Genet. Sel. Evol.
Volume 35, Number Suppl. 1, 2003
Second International Symposium on Candidate Genes for Animal Health
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Page(s) | S157 - S165 | |
DOI | https://doi.org/10.1051/gse:2003024 |
DOI: 10.1051/gse:2003024
Characterization of the porcine CDKN3 gene as a potential candidate for congenital splay leg in piglets
Steffen Maaka, Simone Jaeserta, Karsten Neumannb and Gerhard von Lengerkenaa Institute of Animal Breeding and Husbandry with Veterinary Clinic, Martin-Luther-University Halle-Wittenberg, Adam-Kuckhoff-Str. 35, 06108 Halle, Germany
b Institute of Zoology, Martin-Luther-University Halle-Wittenberg, Domplatz 4, 06108 Halle, Germany
(Accepted 26 February 2003)
Abstract
Congenital splay leg is a hereditary disease observed in newborn
piglets. The etiology and pathogenetic mechanism of the disorder are
still unknown. The gene for cyclin-dependent protein kinase
inhibitor 3 (CDKN3) was identified as a potential candidate
gene in a differential display experiment. We analyzed the gene on
sequence variations and compared its expression in M. biceps femoris
between healthy and affected piglets. Comparative sequencing
of the coding region of three healthy and four splay leg piglets
revealed twelve single nucleotide polymorphisms (SNP) resulting in six
amino acid exchanges in the deduced sequences. However, all
polymorphisms were observed in healthy as well as in splay leg piglets
thus excluding structural differences of the gene as a cause of the
disease. Besides full length transcripts, we found a variety of
aberrantly transcribed cDNA in clones derived from M. biceps femoris
of healthy as well as of splay leg piglets. All alternative
transcripts coexist with normal cDNA. Expression analysis revealed a
trend towards higher values in M. biceps femoris of splay leg
piglets supporting the results obtained from a differential display.
Key words: CDKN3 gene / expression analysis / congenital splay leg / piglet
Correspondence and reprints: Steffen Maak
e-mail: maak@landw.uni-halle.de
© INRA, EDP Sciences 2003