Issue |
Genet. Sel. Evol.
Volume 37, Number 3, May-June 2005
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Page(s) | 339 - 360 | |
DOI | https://doi.org/10.1051/gse:2005005 |
DOI: 10.1051/gse:2005005
Identification and characterization of single nucleotide polymorphisms in 12 chicken growth-correlated genes by denaturing high performance liquid chromatography
Qinghua Niea, Mingming Leia, Jianhua Ouyanga, b, Hua Zenga, Guanfu Yanga and Xiquan Zhangaa Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China
b College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China
(Received 6 May 2004; accepted 17 December 2004)
Abstract -
The genes that are part of the somatotropic axis play a crucial role in the
regulation of growth and development of chickens. The identification of genetic
polymorphisms in these genes will enable the scientist to evaluate the biological
relevance of such polymorphisms and to gain a better understanding of
quantitative traits like growth. In the present study, 75 pairs of primers
were designed and four chicken breeds, significantly differing in growth and
reproduction characteristics, were used to identify single nucleotide
polymorphisms (SNP) using the denaturing high performance liquid
chromatography (DHPLC) technology.
A total of 283 SNP were discovered in 31 897 base pairs (bp) from 12 genes of
the growth hormone (GH), growth hormone receptor (GHR), ghrelin, growth hormone secretagogue
receptor (GHSR), insulin-like growth factor I and II (IGF-I and -II), insulin-like growth factor
binding protein 2 (IGFBP-2), insulin, leptin receptor (LEPR), pituitary-specific transcription
factor-1 (PIT-1), somatostatin (SS), thyroid-stimulating hormone beta subunit
(TSH-). The observed average distances in bp between the SNP in the 5'UTR, coding
regions (non- and synonymous), introns and 3'UTR were 172, 151 (473 and 222),
89 and 141 respectively. Fifteen non-synonymous SNP altered the translated
precursors or mature proteins of GH, GHR, ghrelin, IGFBP-2, PIT-1 and SS. Fifteen indels of no less
than 2 bps and 2 poly (A) polymorphisms were also observed in 9 genes.
Fifty-nine PCR-RFLP markers were found in 11 genes. The SNP discovered in
this study provided suitable markers for association studies of candidate
genes for growth related traits in chickens.
Key words: chickens / genes / SNP / DHPLC
Correspondence and reprints: xqzhang@scau.edu.cn
© INRA, EDP Sciences 2005