Issue |
Genet. Sel. Evol.
Volume 40, Number 2, March-April 2008
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Page(s) | 227 - 239 | |
DOI | https://doi.org/10.1051/gse:2007046 | |
Published online | 27 February 2008 |
DOI: 10.1051/gse:2007046
Expression pattern and polymorphism of three microsatellite markers in the porcine CA3 gene
Jian Wu1, Donghai Zhou2, Changyan Deng3, Yuanzhu Xiong3, Minggang Lei3, Fenge Li3, Siwen Jiang3, Bo Zuo3 and Rong Zheng31 Department of Basic Veterinary Medicine, College of Veterinary Medicine, Huazhong Agriculture University, Wuhan, 430070, P. R. China
2 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agriculture University, Wuhan, 430070, P. R. China
3 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong Agriculture University, Wuhan, 430070, P. R. China
(Received 12 February 2007; accepted 21 August 2007; published online 27 February 2008)
Abstract -
Carbonic anhydrase III (CA3) is an abundant muscle
protein characteristic of adult type-1, slow-twitch, muscle fibres. In order
to further understand the functions of the porcine CA3 protein in muscle,
the temporal and spatial distributions of its gene product were analysed and
the association between the presence of specific polymorphisms and carcass
traits in the pig was also examined. Real-time PCR revealed that the CA3 mRNA
expression showed no differences with age in skeletal muscles from Yorkshire
pigs at postnatal day-1, month-2, and month-4. We provide the first evidence
that CA3 is differentially expressed in the skeletal muscle of Yorkshire and
Meishan pig breeds. In addition, the whole pig genomic DNA sequence of CA3 was
investigated and shown to contain seven exons and six introns. Comparative
sequencing of the gene from three pig breeds revealed the existence of
microsatellite SJ160 in intron 5 and microsatellite SJ158 and a novel
microsatellite marker that includes a tandem repeat of
in intron 4. We also determined the allele number and frequencies of the three loci in
seven pig breeds and found that they are low polymorphic microsatellite
markers. Statistical analysis showed that the CA3 microsatellite polymorphism
was associated with dressing percentage, internal fat rate, carcass length,
rib number and backfat thickness in the pig.
Key words: pig / carbonic anhydrase III / expression pattern / microsatellite polymorphism
Correspondence and reprints: guanjian830@126.com
© INRA, EDP Sciences 2008