Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

François Piumi; Daniel Vaiman; Edmond-Paul Cribiu; Bernard Guérin; Patrice Humblot

doi:doi:10.1051/gse:2001111

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Volume 33 / No 1 (January-February 2001)

Genet. Sel. Evol., 33 1 (2001) 89-98

Abstract

Free Access

Issue		Genet. Sel. Evol. Volume 33, Number 1, January-February 2001


Page(s)		89 - 98
DOI		https://doi.org/10.1051/gse:2001111

DOI: 10.1051/gse:2001111

Genet. Sel. Evol. 33 (2001) 89-98

Specific cytogenetic labeling of bovine spermatozoa bearing X or Y chromosomes using fluorescent in situ hybridization (FISH)

François Piumi^a, Daniel Vaiman^b, Edmond-Paul Cribiu^b, Bernard Guérin^a and Patrice Humblot^a

^a UNCEIA, Services techniques, Maisons-Alfort, France
^b Département de génétique animale, Laboratoire de génétique biochimique et de cytogénétique, Groupe petits ruminants, Institut national de la recherche agronomique, 78352 Jouy-en-Josas, France

(Received 14 August 2000; accepted 24 October 2000)

Abstract
X and Y specific probes were identified in order to apply the fluorescent in situ hybridization (FISH) technique to bovine spermatozoa. For Y chromosome detection, the BRY4a repetitive probe, covering three quarters of the chromosome, was used. For X chromosome detection, a goat Bacterial Artificial Chromosome (BAC) specific to the X chromosome of bovine and goats and giving a strong FISH signal was used. Each probe labeled roughly $45\%$ of sperm cells. The hybridization method will be useful for evaluating the ratio of X- and Y- bearing spermatozoa in a sperm sample and consequently can be used to evaluate the efficiency of sperm sorting by different techniques such as flow cytometry.

Key words: fluorescent in situ hybridization / spermatozoon / X chromosome / Y chromosome / bacterial artificial chromosome

Correspondence and reprints: F. Piumi
e-mail: piumi@biotec.jouy.inra.fr

© INRA, EDP Sciences 2001