Genet. Sel. Evol.
Volume 33, Number 2, March-April 2001
|Page(s)||191 - 200|
Genet. Sel. Evol. 33 (2001) 191-200
Development and assignment of bovine-specific PCR systems for the Texas nomenclature marker genes and isolation of homologous BAC probesMathieu Gautier, Pascal Laurent, Hélène Hayes and André Eggen
Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, 78352 Jouy-en-Josas Cedex, France
(Received 29 September 2000; accepted 12 December 2000)
In 1996, Popescu et al. published the Texas standard nomenclature of the bovine karyotype in which 31 marker genes, already mapped in man, were chosen to permit unambiguous identification and numbering of each bovine chromosome. However, specific PCR systems were not available for each marker gene thus preventing the assignment of part of these markers by somatic cell hybrid analysis. In addition, some difficulties remained with the nomenclature of BTA25, BTA27 and BTA29. In this work, specific PCR systems were developed for each of the marker genes except VIL1 (see results), from either existing bovine or human sequences, and a bovine BAC library was screened to obtain the corresponding BAC clones. These PCR systems were used successfully to confirm the assignment of each marker gene (except for LDHA, see results) by analysis on the INRA hamster-bovine somatic cell hybrid panel. The difficulties observed for LDHA and VIL1 are probably due to the fact that these genes belong to large gene families and therefore suggest that they may not be the most appropriate markers for a standardisation effort. This panel of BACs is available to the scientific community and has served as a basis for the establishment of a revised standard nomenclature of bovine chromosomes.
Key words: bovine / BAC library / cytogenetics / mapping / Texas standard
Correspondence and reprints: André Eggen firstname.lastname@example.org
© INRA, EDP Sciences 2001