Issue |
Genet. Sel. Evol.
Volume 35, Number Suppl. 1, 2003
Second International Symposium on Candidate Genes for Animal Health
|
|
---|---|---|
Page(s) | S99 - S111 | |
DOI | https://doi.org/10.1051/gse:2003019 |
DOI: 10.1051/gse:2003019
Association of INOS, TRAIL,
TGF-
2, TGF-
3,
and IgL genes with response to Salmonella enteritidis in poultry
Massoud Malek and Susan J. Lamont Iowa State University, 2255 Kildee Hall, Department of Animal Science, Ames, Iowa, 50011-3150, USA
(Accepted 26 February 2003)
Abstract
Several candidate genes were selected, based on their critical roles
in the host's response to intracellular bacteria, to study the genetic
control of the chicken response to Salmonella enteritidis
(SE). The candidate genes were: inducible nitric oxide synthase
(INOS), tumor necrosis factor related apoptosis inducing
ligand (TRAIL), transforming growth factor
2
(TGF-
2), transforming growth factor
3
(TGF-
3), and immunoglobulin G light chain
(IgL). Responses to pathogenic SE colonization or to SE
vaccination were measured in the Iowa Salmonella response resource
population (ISRRP). Outbred broiler sires and three diverse, highly
inbred dam lines produced 508 F
1 progeny, which were
evaluated as young chicks for either bacterial load isolated from
spleen or cecum contents after pathogenic SE inoculation, or the
circulating antibody level after SE vaccination. Fragments of each
gene were sequenced from the founder lines of the resource population
to identify genomic sequence variation. Single nucleotide
polymorphisms (SNP) were identified, then PCR-RFLP techniques were
developed to genotype the F
1 resource population. Linear
mixed models were used for statistical analyses. Because the inbred
dam lines always contributed one copy of the same allele, the
heterozygous sire allele effects could be assessed in the F
1
generation. Association analyses revealed significant effects of the
sire allele of TRAIL-StyI on the spleen (
P<0.07) and cecum
(
P<0.0002) SE bacterial load. Significant effects (
P<0.04) were
found on the cecum bacterial load for TGF-
I. Varied and moderate
association was found for SE vaccine antibody response for all genes. This is the first
reported study on the association of SNP in INOS,
TRAIL, TGF-
, TGF-
, and
IgL with the chicken response to SE. Identification of
candidate genes to improve the immune response may be useful for
marker-assisted selection to enhance disease resistance.
Key words: Salmonella / genetic / candidate gene / PCR-RFLP / poultry
Correspondence and reprints: Susan J. Lamont
e-mail: sjlamont@iastate.edu
© INRA, EDP Sciences 2003