Issue |
Genet. Sel. Evol.
Volume 35, Number Suppl. 1, 2003
Second International Symposium on Candidate Genes for Animal Health
|
|
---|---|---|
Page(s) | S113 - S119 | |
DOI | https://doi.org/10.1051/gse:2003020 |
DOI: 10.1051/gse:2003020
Association studies using random and "candidate" microsatellite loci in two infectious goat diseases
Gabriela Obexer-Ruffa, Ursula Sattlera, Dominique Martinezb, Jean-Charles Maillardb, Christophe Chartierc, Nasikat Saitbekovaa, Marie-Louise Glowatzkia and Claude Gaillardaa University of Berne, Institute of Animal Genetics, Nutrition and Housing, Berne, Switzerland
b Cirad-EMVT, Petit-Bourg, Guadeloupe, FWI, Montpellier, France
c Affsa, Laboratoire d'études et de recherches caprines, Niort, France
(Accepted 4 February 2003)
Abstract
We established a set of 30 microsatellites of Bovidae origin for use
in a biodiversity study in Swiss and Creole goats. Additional
microsatellites located within or next to "candidate" genes of
interest, such as cytokine genes (IL4, INF-gamma) and MHC class II
genes (DRB, DYA) were tested in the caprine species in order to detect
possible associations with two infectious caprine
diseases. Microsatellite analysis was undertaken using automated
sequencers (ABI373 & 3100). In the first study, a total of 82
unrelated Creole goats, 37 resistant and 45 susceptible to Heartwater
disease (Cowdriosis) were analysed. In this study, the two
microsatellite loci DRBP1 (MHCII) and BOBT24 (IL4) were positively
associated with disease susceptibility, demonstrating a corrected
P-value of 0.002 and 0.005, respectively. In a second investigation,
we tested 36 goats, naturally infected with the nematode parasite
Trichostrongylus colubriformis. These animals were divided
into a "low" and "high" excreting group on the basis of two
independently recorded fecal egg counts. For this nematode resistance
study, we detected a significant association of one of the alleles of
the microsatellite locus SPS113 with "low" excretion
(resistance). The MHC class II locus DYA (P19), was weakly associated
with susceptibility in both diseases (
). In future
experiments, we will extend the sample size in order to verify the
described associations.
Key words: disease association / microsatellites / goats
Correspondence and reprints: Gabriela Obexer-Ruff
e-mail: gabriela.obexer-ruff@itz.unibe.ch
© INRA, EDP Sciences 2003