Issue |
Genet. Sel. Evol.
Volume 36, Number 4, July-August 2004
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|
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Page(s) | 481 - 486 | |
DOI | https://doi.org/10.1051/gse:2004012 |
DOI: 10.1051/gse:2004012
Note
Genetic variations of the porcine PRKAG3 gene in Chinese indigenous pig breeds
Lu-Sheng Huang, Jun-Wu Ma, Jun Ren, Neng-Shui Ding, Yuan-Mei Guo, Hua-Shui Ai, Lin Li, Li-Hua Zhou and Cong-Ying ChenKey Laboratory for Animal Biotechnology of Jiangxi Province and the Ministry of Agriculture of China, Jiangxi Agricultural University, Nanchang, 330045, P.R. China
(Received 31 October 2003; accepted 25 March 2004)
Abstract
Four missense substitutions (T30N, G52S, V199I and R200Q) in the porcine PRKAG3 gene were considered
as the likely candidate loci affecting meat quality. In this
study, the R200Q substitution was investigated in a sample of 62 individuals from
Hampshire, Chinese Min and Erhualian pigs, and the genetic variations of
T30N, G52S and V199I substitutions were detected in 1505 individuals from 21 Chinese
indigenous breeds, 5 Western commercial pig breeds, and the wild pig. Allele
200R was fixed in Chinese Min and Erhualian pigs. Haplotypes
II-QQ and IV-QQ were not
observed in the Hampshire population, supporting the hypothesis that allele
200Q is tightly linked with allele 199V. Significant differences in allele
frequencies of the three substitutions (T30N, G52S and V199I) between Chinese indigenous
pigs and Western commercial pigs were observed. Obvious high frequencies of
the "favorable" alleles 30T and 52G in terms of meat quality were detected in
Chinese indigenous pigs, which are well known for high meat quality.
However, the frequency of the "favorable" allele 199I, which was reported to have
a greater effect on meat quality in comparison with 30T and 52G, was very low in all
of the Chinese indigenous pigs except for the Min pig. The reasons accounting for
this discrepancy remain to be addressed. The presence of the three
substitutions in purebred Chinese Tibetan pigs indicates that the three
substitutions were ancestral mutations. A novel A/G substitution at position
51 in exon 1 was identified. The results suggest that further studies are
required to investigate the associations of these substitutions in the
PRKAG3 gene with meat quality of Chinese indigenous pigs, and to uncover other
polymorphisms in the PRKAG3 gene with potential effects on meat quality in Chinese
indigenous pigs.
Key words: pig / PRKAG3 gene / meat quality
Correspondence and reprints: Lushenghuang@hotmail.com
© INRA, EDP Sciences 2004