\n This email address is being protected from spambots. You need JavaScript enabled to view it.
), CBLU, University of Leeds
* revised and updated by: Marcus Hennecke, Ross Moore, Herb Swan
* with significant contributions from:
Jens Lippmann, Marek Rouchal, Martin Wilck and others -->
DOI: 10.1051/gse:2000122
Genet. Sel. Evol. 32 (2000) 321-329
A high-density linkage map of the RN region in pigs
Christian Loofta -
Denis Milanb -
Jin-Tae Jeonc -
Sven Paula -
Norbert Reinscha -
Claire Rogel-Gaillardd -
Virginie Reyb -
Valérie Amargerc -
Annie Robicb -
Ernst Kalma -
Patrick Chardond -
Leif Anderssonc
aInstitute of Animal Breeding and Husbandry,
Christian-Albrechts-University, 24098 Kiel, Germany
bLaboratoire de génétique cellulaire,
Institut national de la recherche agronomique,
31326 Castanet-Tolosan, France
cDepartment of Animal Breeding and Genetics,
Swedish University of Agricultural Sciences,
751 24 Uppsala, Sweden
dLaboratoire de radiobiologie et d'étude du génome,
Institut national de la recherche agronomique
/ Centre énergie atomique,
78352 Jouy-en-Josas Cedex, France
(Received 6 December 1999; accepted 10 February 2000)
Abstract:
The porcine RN locus affects muscle glycogen content and meat
quality. We previously mapped the RN locus to chromosome 15.
This study describes the identification of polymorphisms for four
class I and four class II markers located in the RN region.
Resource families were genotyped with F-SSCP markers (fluorescent
single strand conformation polymorphism) and microsatellite markers.
Subsequent multipoint linkage analysis revealed the order
FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906. The
gene order is identical to the previously reported porcine RH map
of the same region. The described map will facilitate positional
cloning of the RN gene.
Keywords:
pig / meat quality / RN gene / genetic mapping
Résumé:
Cartographie génétique de la région de RN chez le porc.
Le gène RN influence le taux de glycogène musculaire et la
qualité de la viande chez le porc. Il a été cartographié sur le
chromosome 15. Cette étude présente la cartographie de quatre
marqueurs SSCP (Polymorphisme de Conformation Simple Brin) et de
quatre microsatellites situés à proximité de RN. L'analyse
multipoint a permis de montrer que chez le porc l'ordre de ces
marqueurs,
FN1-IGFBP5-S1000-S1001-IL8RB-VIL1-RN-Sw936-Sw906, est
identique à celui chez l'homme et la souris. Le positionnement de
RN relativement aux marqueurs proches facilitera l'identification
du gène responsable par clonage positionnel.
Mots clé :
porc / qualité de la viande / gène RN / liaison génétique
Correspondence and reprints: Christian Looft; This email address is being protected from spambots. You need JavaScript enabled to view it.
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