Genet. Sel. Evol. 36 (2004) 105-122
DOI: 10.1051/gse:2003054

A first generation bovine BAC-based physical map

Laurent Schibler^a, Anne Roig^a, Marie-Françoise Mahé^a, Jean-Claude Save^b, Mathieu Gautier^a, Sead Taourit^a, Didier  Boichard^c, André Eggen^a and Edmond P. Cribiu<sup>a

a</sup>  Laboratoire de génétique biochimique et de cytogénétique, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France
^b  Laboratoire de radiobiologie et d'étude du génome, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France
^c  Station de génétique quantitative appliquée, Département de génétique animale, Institut national de la recherche agronomique, Centre de recherche de Jouy, 78352 Jouy-en-Josas Cedex, France

(Received 18 June 2003; accepted 17 October 2003)

Abstract
A first generation clone-based physical map for the bovine genome was constructed combining, fluorescent double digestion fingerprinting and sequence tagged site (STS) marker screening. The BAC clones were selected from an Inra BAC library (105 984 clones) and a part of the CHORI-240 BAC library (26 500 clones). The contigs were anchored using the screening information for a total of 1303 markers (451 microsatellites, 471 genes, 127 EST, and 254 BAC ends). The final map, which consists of 6615 contigs assembled from 100 923 clones, will be a valuable tool for genomic research in ruminants, including targeted marker production, positional cloning or targeted sequencing of regions of specific interest.

Key words: cattle / physical map / BAC

Correspondence and reprints: schibler@jouy.inra.fr

© INRA, EDP Sciences 2004